This course describes and utilizes the theory of chromatographic separations. Gas and liquid chromatography instrumentation, column efficiency, resolution, qualitative and quantitative analysis techniques, and column choices based on applications are discussed. Sample preparation methods relevant to chromatography are described and applied in the laboratory experiments. Students conduct experiments using gas and liquid chromatography instruments. Injection techniques, temperature programming, and gradient elutions are performed. Qualitative and quantitative analyses of prepared and real samples are completed.