This course introduces the fundamental principles of spectroscopy and chromatographic separations as they relate to the instrumental analysis of biological molecules. UV-Visible, fluorescence, IR, NMR and ICP are explained and these spectroscopic techniques are used in both the qualitative and quantitative analyses of unknown samples. The separation processes in gas and liquid chromatography and the basics of mass spectrometry as used in conjunction with LC are explained and these techniques are used in the qualitative and quantitative analyses of unknown samples.